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Week 6 Wrap-Up Assignment: DNA Extraction

Extracting DNA from Fruit

 

Materials

 

· Re-sealable plastic bag

· Two strawberries or other fruit, like bananas, kiwi, blueberries, etc., or onion (strawberries and bananas tend to have a lot of DNA)

· 2 teaspoons dish soap

· ½ cup water

· 1 teaspoon of salt

· Two plastic cups or clear glasses

· Coffee filter or paper towel

· ½ cup COLD rubbing alcohol (use from refrigerator or put in freezer for 30 minutes)

· Toothpick or coffee stirrer or popsicle stick or pencil

Procedure

 

1. Before you start, take a piece of paper, and write your name and student identification on it. You will need it later for the picture you take.

 

2. Pull off any green leaves that might be left on the strawberries. If using another fruit or vegetable, remove the skin and cut into pieces.

 

3. Put the strawberries in the resealable plastic bag and gently smash them with your hands for about two minutes. You need to completely crush the strawberries. If you have another fruit, you may need to blend it.

 

4. In one of the plastic cups or glasses, mix the dish soap, water, and salt together. This is your DNA extraction liquid

 

5. Add two teaspoons of the DNA extraction liquid into the bag with the strawberries.

 

6. Reseal the bag and smash for another minute. Do this very gently so that you do not create too many soap bubbles.

 

7. Place the coffee filter or paper towel inside the mouth of the other cup or glass.

 

8. Open the bag with the strawberry mixture and slowly pour the liquid into the coffee filter. You may have to hold the filter so that it does not drop down into the cup or glass.

 

9. Once you have poured all the liquid into the filter, carefully pick up the filter, twist it just above the liquid, and gently squeeze the remaining liquid into the cup.

 

10. Tilt the cup/glass and very slowly pour the cold alcohol down the inside of the cup. Use as much alcohol as you have strawberry liquid in the cup. Do not mix or stir! This will isolate the DNA.

 

11. Watch for the DNA to clump together on top of the strawberry liquid. It will be a white cloudy substance. Use the toothpick/coffee stirrer/popsicle stick to gently collect some of the DNA.

 

12. Take a picture of the extracted DNA with the paper with your name and student identification on it and submit it in your lab document.

Evidence

 

Include a picture of your extracted DNA with paper with your name and student identification here.

Conclusions

 

1. Doing a search on the internet, what was the purpose of the dish soap? Reword what you find in your own words.

 

Website link (type or copy your website link here):

 

2. What is the purpose of the salt?

 

Website link (type or copy your website link here):

 

3. What substance causes the DNA to precipitate, and why does it precipitate? Explain. Reword what you find in your own words.

 

 

Website link (type or copy your website link here):

4. Briefly explain how well this lab worked and describe any challenges you had.

2

Struggling with where to start this assignment? Follow this guide to tackle your assignment easily!

Step 1: Understand the Purpose of the DNA Extraction Lab

• Explain the importance of DNA extraction in biology and research.
• Describe the techniques and materials used in the experiment.
• Highlight the objective—to isolate and observe DNA from a biological sample.

Step 2: Structure Your Lab Report

1. Title Page

   • Include the lab title, your name, date, and instructor’s name.

2. Abstract (if required)

   • Summarize the experiment, including the purpose, methods, results, and conclusion.

3. Introduction

   • Explain why DNA extraction is important.
   • Provide background on the structure and function of DNA.
   • State the hypothesis or expected outcome.

4. Materials and Methods

   • List all materials (e.g., detergent, salt, ethanol, fruit or cheek cells).
   • Describe the step-by-step procedure used to extract DNA.
   • Ensure the instructions are clear enough for replication.

5. Results

   • Describe the appearance of the extracted DNA.
   • Use tables, charts, or images (if required) to present findings.
   • Compare results with expected outcomes.

6. Discussion

   • Interpret the results: Was the DNA visible?
   • Discuss factors that may have affected the yield (e.g., temperature, sample quality).
   • Relate findings to real-world applications (e.g., forensics, genetics, biotechnology).

7. Conclusion

   • Summarize key findings and state whether the experiment was successful.
   • Suggest improvements for future experiments.

8. References (if required)

   • Cite scientific sources in APA or the required format.

Step 3: Formatting and Submission

• Follow the required word count and citation style.
• Ensure clarity, conciseness, and accuracy.

By structuring your report this way, you’ll create a strong, well-organized lab assignment!

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